PHYLOGENETIC ASSESSMENT OF TREE SPECIES OF ACONITUM L. FROM KAZAKHSTAN BY USING ITS AND MATK MARKERS

  • Sh. Almerekova Institute of Plant Biology and Biotechnology, Al-Farabi Kazakh National University
  • A. Ivaschenko Ile-Alatau State Nature Reserve
  • R. Kaparbay Institute of Plant Biology and Biotechnology
  • A. Myrzagalieva Astana International University
  • Ye. Turuspekov Institute of Plant Biology and Biotechnology, Al-Farabi Kazakh National University
DOI: https://doi.org/10.31618/ESU.2413-9335.2020.2.79.1036
Keywords: Aconitum, DNA barcoding, ITS, matK, phylogenetics, haplotype network

Abstract

The Aconitum L. is a diverse genus consisting of more than 300 species all over the World, including 11 species grown in Kazakhstan. The phylogeny of the genus was mostly studied by using internal transcribed sequences (ITS) of the nuclear genome and maturase K (matK) of the chloroplast genome. Therefore, in this study it was decided to assess the phylogenetic position of three local species A. leucostomum, A. soongoricum and A. apetalum, by using ITS and matK. The application of Maximum-Likelihood (ML) method suggested that the A. soongoricum belong to subgenus Aconitum, and A. leucostomum and A. apetalum to the subgenus Lycoctonum, which was congruent to previous taxanomic studies for this genus. The Median-Joining network using ITS suggested that A. sachalinense belongs to the group of processors of the genus among species that were involved in the analysis. The study is the first attempt to understand phylogenetic relationship of three species of Aconitum grown in Kazakhstan. 

Author Biographies

Sh. Almerekova , Institute of Plant Biology and Biotechnology, Al-Farabi Kazakh National University

Biodiversity and Bioresources Department

 

Ye. Turuspekov, Institute of Plant Biology and Biotechnology, Al-Farabi Kazakh National University

Biodiversity and Bioresources Department

References

1. Tamura M. Ranunculaceae In: Hiepko P, editor //Die Naturlichen Pflanzenfamilien. – 1995. – V. 17. – №. 4. – P. 223-555.
2. Kita Y, Ueda K, Kadota Y. Molecular Phylogeny and Evolution of the Asian Aconitum Subgenus Aconitum (Ranunculaceae). J. Plant Res. 1995;08(4):429-442. https://doi.org/10.1007/BF02344231
3. Hong Y, Luo Y, Gao Q, et al. Phylogeny and reclassification of Aconitum subgenus Lycoctonum (Ranunculaceae). PLoS One. 2017;12(1):e0171038. https://doi.org/10.1371/journal.pone.0171038
4. Luo Y, Zhang FM, Yang Q-E. Phylogeny of Aconitum subgenus Aconitum (Ranunculaceae) inferred from ITS sequences. Plant Syst. Evol. 2005;252: 11–25. https://doi.org/10.1007/s00606-004-0257-5
5. Tamura M. A new classification of the family Ranunculaceae 1. Acta Phytotax. Geobot. 1990;41:93–101.
6. Kita Y, Ito M. Nuclear ribosomal ITS sequences and phylogeny in East AsianAconitum subgenusAconitum (Ranunculaceae), with special reference to extensive polymorphism in individual plants. Plant Syst. Evol. 2000;225(1-4):1-13. https://doi.org/10.1007/BF00985455
7. He J, Wong KL, Shaw PC., et al. Identification of the Medicinal Plants in Aconitum L. by DNA Barcoding Technique. Planta med. 2010;76(14): 1622-1628. http://dx.doi.org/10.1055/s-0029-1240967
8. Abdulina SA Checklist of vascular plants of Kazakhstan. Edited by R.V. Kamelin. Almaty: Academy of Sciences of Kazakhstan; 1999.
9. Грудзинская Л.М., Гемеджиева Н.Г., Нелина Н.В., Каржаубекова Ж.Ж. Аннотированный список лекарственных растений Казахстана: Cправочное издание. Алматы; 2014.[Grudzinskaya LM, Gemedzhieva NG, Nelina NV, Karzhaubekova JJ. Annotated checklist of medicinal plants in Kazakhstan: a reference book. Almaty; 2014. (In Russ).]
10. Hebert PD, Cywinska A, Ball SL, et al. Biological identifications through DNA barcodes. Proc. Biol. Sci. 2003;270(1512):313–321. https://doi.org/10.1098/rspb.2002.2218
11. Pang X, Song J, Zhu Y, et al. Applying plant DNA barcodes for Rosaceae species identification. Cladistics. 2011;27(2):165–170. https://doi.org/10.1111/j.1096-0031.2010.00328.x
12. Gao T, Yao H, Song J, et al. Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2. J. Ethnopharmacol. 2010;130(1):116-121. https://doi.org/10.1016/j.jep.2010.04.026
13. Ma HL, Zhu ZB, Zhang XM, et al. Species identification of the medicinal plant Tulipa edulis (Liliaceae) by DNA barcode marker. Biochem. Syst. Ecol. 2014;55:362–368. https://doi.org/10.1016/j.bse.2014.03.038
14. Group C. P. W. et al. A DNA barcode for land plants. PNAS USA. 2009;106(31):12794-12797. https://doi.org/10.1073/pnas.0905845106
15. Group C. P. B. O. L. et al. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. PNAS USA. 2011;108(49):19641-19646. https://doi.org/10.1073/pnas.1104551108
16. Alvarez I, Wendel JF. Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution. 2003;29(3):417–434. https://doi.org/10.1016/S1055-7903(03)00208-2
17. Cheng T, Xu C, Lei L, et al. Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Mol. Ecol. Resour. 2016;16(1):138-149. https://doi.org/10.1111/1755-0998.12438
18. Gültepe M, Uzuner U, Coşkunçelebi K, et al. Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turk J Botany. 2010;34(3):147-157. doi: 10.3906/bot-0905-23
19. Dellaporta SL, Wood J, Hicks JB. A plant DNA mini preparation: Version II. Plant. Mol. Biol. Rep. 1983;1:19–21.
20. Hollingsworth PM, Graham SW, Little DP. Choosing and Using a Plant DNA Barcode. PLoS ONE. 2011;6(5):e19254. https://doi.org/10.1371/journal.pone.0019254
21. Jun W, Nian-He X. Ardisia crenata complex (Primulaceae) studies using morphological and molecular data. Botany. 2012;163-172.
22. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 2016;33:1870-1874. https://doi.org/10.1093/molbev/msw054
23. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 1993;10:512-526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
24. Librado P, Rozas J. DnaSP v5 a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009;25(11):1451-1452. https://doi.org/10.1093/bioinformatics/btp187
25. Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol. Evol. 2015;6(9):1110-1116. doi: 10.1111/2041-210X.12410
26. Chan TYK. Aconite poisoning. Clinical toxicology. 2009;47(4):279-285. https://doi.org/10.1080/15563650902904407
27. Xiao PG, Wang FP, Gao F, et al. A pharmacophylogenetic study of Aconitum L. (Ranunculaceae) from China. Acta Phytotax. Sin. 2006;44 (1):1-46. doi: 10.1360/aps050046
28. Kress WJ, Wurdack KJ, Zimmer EA, et al. Use of DNA barcodes to identify flowering plants. PNAS USA. 2005;102(23):8369-8374. https://doi.org/10.1073/pnas.0503123102
Published
2020-11-20
How to Cite
Almerekova , Sh., A. Ivaschenko, R. Kaparbay, A. Myrzagalieva, and Ye. Turuspekov. 2020. “PHYLOGENETIC ASSESSMENT OF TREE SPECIES OF ACONITUM L. FROM KAZAKHSTAN BY USING ITS AND MATK MARKERS ”. EurasianUnionScientists 2 (10(79), 4-9. https://doi.org/10.31618/ESU.2413-9335.2020.2.79.1036.
Issue
Vol. 2 No. 10(79) (2020): EURASIAN UNION OF SCIENTISTS №79
Section
Article

CC BY-ND

A work licensed in this way allows the following:

1. The freedom to use and perform the work: The licensee must be allowed to make any use, private or public, of the work.

2. The freedom to study the work and apply the information: The licensee must be allowed to examine the work and to use the knowledge gained from the work in any way. The license may not, for example, restrict "reverse engineering."

2. The freedom to redistribute copies: Copies may be sold, swapped or given away for free, in the same form as the original.