PHILOGENETIC DIVERSITY OF FUNGI OF THE GENUS PENICILLIUM AND TALAROMYCES ASSOCIATED WITH BROWN ALGAE OF THE GENUS SARGASSUM
Abstract
The results of the investigation of Penicillium sensu lato phylogenetic diversity of brown algae Sargassum spp. (the Sea of Japan) are presented in this article. Seventeen fungal strains were studied using traditional methods of phenotypic investigations and modern molecular-genetic approaches. As a result of phylogenetic analysis of ITS and BenA gene sequences, Penicillium species from five sections were revealed (sections Fasciculata, Paradoxa, Ramosa, Canescentia, Aspergilloides). One strain was identified as species of Talaromyces which more related to section Talaromyces. Five species (P. spinulosum, P. subspinulosum, P. roseomaculatum, P. thomii, P. murcianum, P. antarcticum) were identified based on BenA gene analysis. For species identification of some strains additional analysis of genetic and phenotypic features is necessary.
References
2.Dewey FM, Donnelley KA, Foster D Penicillium waksmanii isolated from a red seaweed, Eucheuma striatum. Trans. Br. Mycol. Soc. 1983;81:433-434.
3.Sterflinger K, Hain WM, Scholz OJ, et al. Fungal infections of a colonial marine invertebrate: diversity and morphological consequences. FACIES. 2001;45:31-38.
4.Слинкина Н.Н., Пивкин М.В. Биоразнообразие грибов аквапочв южной части Сахалина // Микология и фитопатология. 2007. Т. 41, № 1. С. 48-56. [Slinkina NN, Pivkin MV Fungal biodiversity of submarine soils from south part of Sakhalin island. Mikologija i fitopatologija [Mycology and phytopathology]. 2007;41(1):48-56. (in Russ).]
5.Слинкина Н.Н., Пивкин М.В., Полохин О.В. Мицелиальные грибы аквапочв Сахалинского залива (Охотское море)//Биол. моря. 2010. Т. 36, № 6. С. 410-414. [Slinkina NN, Pivkin MV, Polokhin OV Filamentous Fungi of the Submarine Soils of the Sakhalin Gulf (Sea of Okhotsk). Russian Journal of Marine Biology. 2010;36(6):413-418. (in Russ).] https://doi.org/10.1134/S1063074010060027
6.Киричук Н.Н., Пивкин М.В., Полохин О.В. Грибные комплексы акваземов восточносахалинского шельфа//Биол. моря. 2012. Т. 38, № 5, С. 363-369. [Kirichuk NN, Pivkin MV, Polokhin OV Fungal Assemblages of Submarine Soils of the Eastern Sakhalin Shelf. Russian Journal of Marine Biology. 2012;38(5):375-380. (in Russ).] https://doi.org/10.1134/S1063074012050069
7.Hyde KD A study of the vertical zonation of intertidal fungi on Rhizophora spp. at Kampung Danau mangrove. 1988;36:255-262.
8.Жирков И.А. Жизнь на дне. Биогеография и биоэкология бентоса. М.; 2010. [Zhirkov IA Zhizn' na dne. Biogeografiya i bioecologiya bentosa. M.; 2010. (in Russ).]
9.Ding L, Qin S, Li F, et al. Isolation, antimicrobial activity, and metabolites of fungus Cladosporium sp. associated with red alga Porphyra yezoensis. Curr. Microbiol. 2008;56:229-235.
10.Пивкин М.В., Кузнецова Т.А., Сова В.В. Морские грибы и их метаболиты. Владивосток: Дальнаука; 2006. [Pivkin MV, Kuznecova TA, Sova VV Marine fungi and their metabolites. Vladivostok: Dal'nauka; 2006. (in Russ).]
11.Киричук Н.Н., Пивкин М.В. Вторичные морские грибы, ассоциированные с бурыми водорослями рода Sargassum залива Петра
Великого//Микология и фитопатология. 2015. Т. 49, № 3. С. 146-150. [Kirichuk NN, Pivkin MV, Secondary marine fungi associated with brown algae Sargassum spp. from Peter the Great Bay (Sea of Japan).Mikologija i fitopatologija [Mycology and phytopathology]. 2015;49:146-150. (in Russ).] 12.Зверева Л.В. Микобиота культивируемой бурой водоросли Laminaria japonica//Биол. моря. 1998. Т. 24, № 1. С. 19-23. [Zvereva LV Mycobiota of cultivated brown algae Laminaria japonica. Russian Journal of Marine Biology.1998;24(1):19-23. (in Russ).]
13.Zuccaro A, Schoch C, Spatafora J, Kohlmeyer J, Draeger S, Mitchell J Detection and identification of fungi intimately associated with the brown seaweed Fucus serratus. Appl. Environ. Microbiol. 2008;74:931-941.
14.Литвинов М.А., Дудка И.А. Методы исследования микроскопических грибов пресных и соленых (морских) водоемов. Л.: Наука; 1975. [Litvinov MA, Dudka IA Metody issledovaniya mikroskopicheskikh gribov presnykh I solenykh (morskikh) vodoemov. L.: Nauka; 1975. (in Russ).] 15.Visagie CM, Houbraken J, Frisvad JC, et al. Identification and nomenclature of the genus Penicillium. Studies in Mycology. 2014;78:343–371. https://doi.org/10.1016/j.simyco.2014.09.001
16.White TJ, Bruns T, Lee S, Taylor JW Amplificaion and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A Guide to Methods and Applications. London, UK: Academic Press; 1990.
17.Glass NL, Donaldson GC Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61:1323-1330.
18.Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony УДК: 579.8.06 ГРНТИ: 34.27: микробиология Methods. Molecular
Biology and Evolution. 2011;28(10):2731-2739. https://doi.org/10.1093/molbev/msr121
19.Tamura K and Nei M Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution. 1993;10:512-526.
20.Felsenstein J Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985;39:783-791.
21.McRae CF, Hocking AD, Seppelt RD Penicillium species from terrestrial habitats in the Windmill Island, East Antarctica, including a new species, Penicillium antarcticum. Polar Biol. 1999;21:97-111. https://doi.org/10.1007/s003000050340
22.Park MS, Lee EJ, Fong JJ, Sohn JH, Lim YW A new record of Penicillium antarcticum from marine environments in Korea. Mycobiology. 2014;42:109-113. https://doi.org/10.1007/s12275-015-4700-9
23.Yilmaz N, Visagie CM, Houbraken J et al. Polyphasic taxonomy of the genus Talaromyces. Studies in Mycology. 2014;78:175–341. http://dx.doi.org/10.1016/j.simyco.2014.08.001
CC BY-ND
A work licensed in this way allows the following:
1. The freedom to use and perform the work: The licensee must be allowed to make any use, private or public, of the work.
2. The freedom to study the work and apply the information: The licensee must be allowed to examine the work and to use the knowledge gained from the work in any way. The license may not, for example, restrict "reverse engineering."
2. The freedom to redistribute copies: Copies may be sold, swapped or given away for free, in the same form as the original.